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Catalog Number | orb390343 |
---|---|
Category | Tools |
Description | Stem-loop RT primer and PCR primer set to detect mature miRNA hsa-mir-521 expression. |
Tested applications | PCR |
Target | hsa-mir-521 RT-PCR Primer Set |
Sensitivity | 10 copies of a standard mimics and as little as 25 pg of total RNA |
Storage | Shipped at 4°C. Store at -20°C for 2 Years. |
Note | For research use only |
Application notes | The miRNAs RT-PCR primer set contains a stem-loop RT primer and a pair of specific PCR primer set. Combined with the custom's RT and PCR reagent. It provides a sensitive and specific method of real-time PCR for quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set. Experiment Notes: PCR Method: 2-Step qRT-PCR Detection Range: Seven orders of magnitude Sample Types: miRNA, Total RNAPlatform: ABI 7500 System/ViiA™ 7 System/StepOne™/StepOnePlus™/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480 |
Expiration Date | 12 months from date of receipt. |
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