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hsa-mir-34a Real-Time RT-PCR Detection and U6 Calibration Kit
Catalog Number: orb220320
|Product Name||hsa-mir-34a Real-Time RT-PCR Detection and U6 Calibration Kit|
|Sensitivity||10 copies of a standard mimics and as little as 25 pg of total RNA|
|Storage||Store at 4°C for short term and at -20°C for long term storage|
|Note||For research use only.|
|Kit Components||50 rxns: It contains all reagents required to perform 50 cDNA reactions and 50 PCR reactions.|
100 rxns: It contains all reagents required to perform 100 cDNA reactions and 100 PCR reactions.
200 rxns: It contains all reagents required to perform 200 cDNA reactions and 200 PCR reactions.
Detect mature miRNA hsa-mir-34a expression normalized by U6 snRNA.
|Application Notes||50 reactions for Real-Time PCR|
Experiment Notes: The Real-Time RT-PCR detection and U6 calibration Kit contains the whole reagent including the stem-loop RT primer & specific PCR primer set for microRNA quantificaion and U6 primer set as housekeeping gene for calibration. It provides a sensitive and specific method of real-time PCR for relative quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set.
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