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hsa-mir-34a Real-Time RT-PCR Detection and U6 Calibration Kit

Catalog Number: orb220320

Product Overview
Product Name hsa-mir-34a Real-Time RT-PCR Detection and U6 Calibration Kit
Catalog Number orb220320
ReactivityHuman
Sensitivity 10 copies of a standard mimics and as little as 25 pg of total RNA
Tested applicationsPCR
Target hsa-mir-34a
Product Properties
Storage Store at 4°C for short term and at -20°C for long term storage
Note For research use only.
Kit Components 50 rxns: It contains all reagents required to perform 50 cDNA reactions and 50 PCR reactions.
100 rxns: It contains all reagents required to perform 100 cDNA reactions and 100 PCR reactions.
200 rxns: It contains all reagents required to perform 200 cDNA reactions and 200 PCR reactions.
Product Description

Detect mature miRNA hsa-mir-34a expression normalized by U6 snRNA.

Application Notes
Application Notes 50 reactions for Real-Time PCR

Experiment Notes: The Real-Time RT-PCR detection and U6 calibration Kit contains the whole reagent including the stem-loop RT primer & specific PCR primer set for microRNA quantificaion and U6 primer set as housekeeping gene for calibration. It provides a sensitive and specific method of real-time PCR for relative quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set.
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