hsa-mir-1271-5p Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit [Out of stock]

• Catalog Number: orb390601
• Category: Tools
• Description: Detect mature miRNA hsa-mir-1271-5p expression normalized by cel-mir-39-3p.
• Tested applications: PCR
• Target: hsa-mir-1271-5p Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
• Sensitivity: 10 copies of a standard mimics and as little as 25 pg of total RNA
• Storage: Shipped at 4°C. Store at -20°C for 6 months.
• Note: For research use only
• Application notes: The Real-Time RT-PCR detection and cel-mir-39-3p calibration Kit contains the whole reagent including the stem-loop RT primer & specific PCR primer set for microRNA quantificaion and cel-mir-39-3p primer set for calibration. It provides a sensitive and specific method of real-time PCR for relative quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set.; ; Experiment Notes: PCR Method: 2-Step qRT-PCR; Detection Range: Seven orders of magnitude; Sample Types: miRNA, Total RNAPlatform: ABI 7500 System/ViiA™ 7 System/StepOne™/StepOnePlus™/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480Components: ; 50 rxns: It contains all reagents required to perform 50 cDNA reactions and 50 PCR reactions.; 100 rxns: It contains all reagents required to perform 100 cDNA reactions and 100 PCR reactions.; 200 rxns: It contains all reagents required to perform 200 cDNA reactions and 200 PCR reactions.
• Expiration Date: 12 months from date of receipt.