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hsa-mir-1178 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit [Out of stock]

hsa-mir-1178 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit [Out of stock]

Catalog Number: orb390600

DispatchUsually dispatched within 5-10 working days
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Catalog Numberorb390600
CategoryTools
DescriptionDetect mature miRNA hsa-mir-1178 expression normalized by cel-mir-39-3p.
Tested applicationsPCR
Targethsa-mir-1178 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
Sensitivity10 copies of a standard mimics and as little as 25 pg of total RNA
StorageShipped at 4°C. Store at -20°C for 6 months.
NoteFor research use only
Application notesThe Real-Time RT-PCR detection and cel-mir-39-3p calibration Kit contains the whole reagent including the stem-loop RT primer & specific PCR primer set for microRNA quantificaion and cel-mir-39-3p primer set for calibration. It provides a sensitive and specific method of real-time PCR for relative quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set.

Experiment Notes: PCR Method: 2-Step qRT-PCR
Detection Range: Seven orders of magnitude
Sample Types: miRNA, Total RNAPlatform: ABI 7500 System/ViiA™ 7 System/StepOne™/StepOnePlus™/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480Components:
50 rxns: It contains all reagents required to perform 50 cDNA reactions and 50 PCR reactions.
100 rxns: It contains all reagents required to perform 100 cDNA reactions and 100 PCR reactions.
200 rxns: It contains all reagents required to perform 200 cDNA reactions and 200 PCR reactions.
Expiration Date12 months from date of receipt.
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