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Product Overview
Product Name HPV16 E6 protein antibody
Catalog Number orb10837
Species/HostRabbit
ReactivityHuman
ConjugationUnconjugated
Tested applicationsELISA, IF, IHC-P
Immunogen

KLH conjugated synthetic peptide derived between 95-148 amino acids of human HPV16-E6/E6

Target HPV16 E6
Alternative Names
Product Properties
Form/Appearance Liquid: 0.01M TBS (pH7.4), 0.5% BSA, 0.015% ProClin™ 300 and 25% glycerol
Concentration

- 5 μg (in 100 μl): 0.05 mg/ml
- 20 μg (in 100 μl): 0.2 mg/ml
- 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml

Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity Affinity purified by Protein A
MW 11 kDa
NCBI NP_041325.11; NC_001526.21
Product Description

Rabbit polyclonal antibody to HPV16-E6/E6 and is also known as protein E6 whose expression can be detected in the early protein. It is found in the host cytoplasm. This protein plays a major role with other cellular targets including BAK1, Fas-associated death domain-containing protein (FADD) and procaspase 8, are degraded by E6/E6AP causing inhibition of apoptosis and in the induction and maintenance of cellular transformation. The complex E6/E6AP targets several other substrates to degradation via the proteasome including host DLG1 or NFX1, a repressor of human telomerase reverse transcriptase (hTERT).

Application Notes
Dilution Range ELISA: 1:500-1000, IHC-P: 1:400-800, IF: 1:100-500
Validation Images
Immunohistochemical analysis of formalin fixed and paraffin embedded human tumor tissue using HPV16-E6/E6 antibody
Immunohistochemical analysis of formalin fixed and paraffin embedded human tumor tissue using HPV16-E6/E6 antibody
Immunohistochemical analysis of formalin fixed and paraffin embedded human cervical carcinoma using HPV16 E6 protein antibody
Immunohistochemical analysis of formalin fixed and paraffin embedded human cervical carcinoma using HPV16 E6 protein antibody
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