HMBS antibody

• Catalog Number: orb318745
• Category: Antibodies
• Description: Goat polyclonal antibody to HMBS
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 39.3; 37.7; 34.9; 33.3
• Target: HMBS (aa160-173)
• Entrez: 3145
• Protein Sequence: HLEFRSIRGNLNTR
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti HMBS antibody, anti hydroxymethylbilane synth
• Note: For research use only
• Application notes: WB: Approx 40kDa band observed in lysates of cell line K562 (calculated MW of 39.3kDa according to NP_000181.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of K562 lysate using HMBS antibody

Immunohistochemical staining of Human Spleen using HMBS antibody