HLA-G antibody

• Catalog Number: orb154378
• Category: Antibodies
• Description: Mouse monoclonal antibody to HLA-G which is a member of the MHC class I family that is observed in trophoblasts. It involved in the presentation of foreign antigens to the immune system also participate in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
• Clonality: Monoclonal
• Clone Number: 87G
• Tested applications: ELISA, FC, IHC-Fr
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Extracellular and intracellular staining; Recommended dilution: 2 μg/ml; positive control: JEG-3 human choriocarcinoma epithelial cell line. Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/ml, incubation: 20 min at 25°C; positive tissue: extravillous cytotrophoblast. ELISA: Recommended dilution: 1 μg/ml; positive control: JEG-3 human choriocarcinoma epithelial cell line. The antibody 87G has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody W6/32.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: HLA-G
• Entrez: 3135
• UniProt ID: P17693
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Note: For research use only
• Application notes: Flow cytometry: Extracellular and intracellular staining; Recommended dilution: 2 μg/ml; positive control: JEG-3 human choriocarcinoma epithelial cell line. Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/ml, incubation: 20 min at 25°C; positive tissue: extravillous cytotrophoblast. ELISA: Recommended dilution: 1 μg/ml; positive control: JEG-3 human choriocarcinoma epithelial cell line. The antibody 87G has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody W6/32.
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of human polyclonal NK cells using HLA-G antibody