HLA-A antibody

• Catalog Number: orb137660
• Category: Antibodies
• Description: HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HC10
• Tested applications: ELISA, FC, ICC, IHC, IM, IP, WB
• Reactivity: Human
• Isotype: IgG2a
• Form/Appearance: Each vial contains 100 µl 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Conjugation: Unconjugated
• Source: HC10 is a mouse monoclonal IgG2a antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with HLA-B7 and -B40 heavy chains.
• Storage: Storage: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product
• Buffer/Preservatives: Each vial contains 100 µl 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry staining of human tonsil tissue using HLA-A antibody.