HLA-A antibody (Biotin)

• Catalog Number: orb44577
• Category: Antibodies
• Description: Mouse monoclonal antibody against HLA-A conjugated to Biotin
• Clonality: Monoclonal
• Clone Number: W6/32
• Tested applications: ELISA, FC, ICC, IHC-Fr, IP
• Reactivity: Bovine, Feline, Human, Primate
• Isotype: Mouse IgG2a
• Immunogen: Membrane of human tonsil cells
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-12 µg/ml
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: HLA Class I
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-HLA A Antibody, anti-HLA B Antibody, anti-HLA
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-12 µg/ml
• Expiration Date: 12 months from date of receipt.

Separation of human leukocytes (red-filled) from HLA Class I negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human HLA Class I (W6/32) Biotin antibody (concentration in sample 4 μg/ml, Streptavidin APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA Class I (W6/32) Biotin antibody (concentration in sample 4 μg/ml, Streptavidin APC).