Histone H1 antibody

• Catalog Number: orb765398
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H1. AA range:1-50
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 31 28
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-HIST1H1B antibody, anti-H1F5 antibody, anti-H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of Hela cell. 1, Histone H1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). α-SMA Monoclonal Antibody (6A12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western Blot analysis of various cells using Histone H1 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Western Blot analysis of 3T3 cells using Histone H1 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Immunofluorescence analysis of HUVEC cells, using Histone H1 Antibody. The picture on the right is blocked with the synthesized peptide.