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Histone H1 Antibody

Catalog Number: orb606517

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb606517
CategoryAntibodies
DescriptionEukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Species/HostMouse
ClonalityRecombinant
Clone Numberr1415-1
Tested applicationsFACS, IF, IHC-P, WB
ReactivityHuman, Mouse, Rat
IsotypeMouse IgG2a, kappa
ImmunogenNuclei of human leukemia biopsy cells were used as the immunogen for the recombinant Histone H1 antibody.
Antibody TypePrimary Antibody
Dilution rangeFlow cytometry: 1-2ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis recombinant Histone H1 antibody is available for research use only.
UniProt IDP07305
StorageStore the recombinant Histone H1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the recombinant Histone H1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.2. View the non-recombinant version of this Histone H1 antibody.
Expiration Date12 months from date of receipt.
Histone H1 Antibody

IHC testing of FFPE human tonsil with recombinant Histone H1 antibody (clone r1415-1). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Histone H1 Antibody

IHC testing of FFPE rat pancreas with recombinant Histone H1 antibody (clone r1415-1). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Histone H1 Antibody

SDS-PAGE analysis of purified, BSA-free recombinant Histone H1 antibody (clone r1415-1) as confirmation of integrity and purity.

Histone H1 Antibody

Flow cytometry testing of permeabilzed human HeLa cells with recombinant Histone H1 antibody (clone r1415-1); Red = isotype control, Blue = recombinant Histone H1 antibody.

Histone H1 Antibody

Immunofluorescent staining of PFA-fixed human HeLa cells with recombinant Histone H1 antibody (clone r1415-1, green) and Phalloidin (red).

Histone H1 Antibody

Western blot testing of human heart lysate with recombinant Histone H1 (clone R1415-1). Observed molecular weight ~22/30-33 kDa (unmodified/modified).

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