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Hirudin protein
Catalog Number: orb80174
| Catalog Number | orb80174 |
|---|---|
| Category | Proteins |
| Description | Recombinant Hirudin is derived from yeast and the polypeptide chain contains 65 amino acids and its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule and the absence of sulfate group on the tyrosine at position 63.The Recombinant Hirudin is purified by proprietary chromatographic techniques. |
| Tested applications | FA, HPLC, SDS-PAGE |
| Concentration | 0.5 mg/ml; 100 ul |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Conjugation | Unconjugated |
| MW | 6.7 kDa |
| Target | Hirudin |
| Protein Sequence | VVYTDCTESG QNLCLCEGSN VCGQGNKCIL GSDGEKNQCV TGEGTPGPQS HNDGDFEEPE EYL |
| Biological Origin | Pichia Pastoris |
| Biological Activity | The biological activity is measured using chromogenic assay, unit is defined as the amount of Hirudin that neutralizes unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. |
| Endotoxins | Less than 1 EU/μg of rHirudin as determined by LAL method. |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of 20 mM PBS, pH 7.0, containing 2 % mannitol. |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
Hirudin protein SDS-PAGE
- Item 1 of 1
Animal Hirudin variant-1 protein [orb383017]
Greater than 90% as determined by SDS-PAGE.
9 kDa
Yeast
1 mg, 20 μg, 100 μg - Item 1 of 1
Virus Hirudo medicinalis Hirudin variant-1 protein [orb604805]
Greater than 90% as determined by SDS-PAGE.
34 kDa
E.coli
20 μg, 100 μg, 1 mg 
Hirudin Protein [orb168498]
Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Pichia Pastoris
2 μg, 10 μg, 1 mg
