HER2 antibody

• Catalog Number: orb256519
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HER2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, IP, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1221/Y1222 of human HER2 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200, IP: 1:10-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ERBB2
• Entrez: 2064
• UniProt ID: P04626
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-CD340 antibody, anti- ERBB2 antibody, anti- H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HER2 (Phospho-Y1221/Y1222) expression in HepG2 EGF-treated (A), A431 EGF-treated (B) whole cell lysates. (Predicted band size: 137 kD; Observed band size: 185 kD)

Immunohistochemical analysis of HER2 (Phospho-Y1221/Y1222) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.