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HEK293 Whole Cell Lysate MG-132 Treated

Catalog Number: orb420146

Product Overview
Product Name HEK293 Whole Cell Lysate MG-132 Treated
Catalog Number orb420146
Hazard InformationNon-Toxic
Target HEK293 Whole Cell Lysate MG-132 Treated
Product Properties
Form/Appearance Liquid: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Concentration 1mg/mL
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Product Description

HEK293 Whole Cell Lysate MG-132 Treated

Application Notes
Application Notes Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 μl depending on the size format of your gel.
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