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Catalog Number | orb668903 |
---|---|
Category | Proteins |
Description | It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
Reactivity | Virus |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 58.8 kDa |
Target | Spike S1 |
Protein Sequence | Cys 16 - Ala 536 |
Source | HCoV-229E S1 protein, His Tag (orb668903) is expressed from human 293 cells (HEK293). It contains AA Cys 16 - Ala 536 (Accession # P15423-1). |
Expression System | HEK293 |
Expression Region | Cys 16 - Ala 536 |
Endotoxins | 1.0 EU per μg |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | Spike,S1 protein,Spike glycoprotein Subunit1,S gly Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 58.8 kDa. The protein migrates as 70-110 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation. |
Expiration Date | 6 months from date of receipt. |
WB | |
> 90% as determined by SDS-PAGE. | |
58.8 kDa | |
HEK293 cells |
98% | |
Approxiamtely 122.1 kDa |
87.70% | |
Approxiamtely 122.1 kDa |
88.50% | |
Approxiamtely 58.3 kDa |
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