hCG alpha Antibody / Luteinizing Hormone alpha

• Catalog Number: orb248303
• Category: Antibodies
• Description: This antibody reacts with a protein of ~13kDa, identified as alpha sub-unit of HCG. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. HCG is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HCGa/53
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant protein was used as the immunogen for this hCG alpha antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (HIER is not recommended for this antibody)
• Purity: Protein G purified monoclonal antibody
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This hCG alpha antibody is available for research use only.
• UniProt ID: P01215
• Storage: Store the hCG alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Variations in protocols, secondaries and substrates may require the hCG alpha antibody to be titered for optimal performance.1. No pretreatment required for FFPE section testing. HIER is not recommended.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human placenta tissue using HCG-alpha antibody