Haptoglobin antibody

Catalog Number: orb21972

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CategoryPrimary antibodies
Catalog Numberorb21972
Species/HostGoat
ClonalityPolyclonal
Tested applicationsDOT, IEP
ReactivityPorcine
IsotypeIgG
ConcentrationTotal protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
ConjugationUnconjugated
UniProt IDQ8SPS7
Application notesThe antiserum does not cross-react with any other component of swine plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion against several sera with the following results: bovine - cat - chicken - dog - duck - guinea pig - hamster - horse - human - monkey - mouse - pigeon - rat - sheep - turkey-IE: In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ulin a 3 mm diameter center well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
StorageThe lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
DescriptionGoat polyclonal antibody to Haptoglobin.
Alternative namesanti Haptoglobin alpha chain antibody, anti Haptoglobin alpha(1S) beta antibody, anti Haptoglobin alpha(2FS) beta antibody, anti Haptoglobin beta chain antibody, anti Haptoglobin, alpha polypeptide antibody, anti Haptoglobin, beta polypeptide antibody, anti HP antibody, anti Hp2 alpha antibody, anti HPA1S antibody, anti HPT antibody, anti Zonulin antibody(click to expand)
Expiration Date12 months from date of receipt.
NoteFor research use only.
SourceHaptoglobin is an acute phase protein synthesized in the liver. In immunoelectrophoresis it has an alpha-2 mobility. It forms a group of heterogeneous proteins consisting of four peptide chains, two alpha and two beta chains. The molecular weight is about 86,000. Haptoglobin combines with haemoglobin and is then rapidly catabolized. Concentration is elevated in cases of inflammation and reduced in liver disease and haemolytic anaemia. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  1. 5 images
    • Immunohistochemical analysis of formalin-fixed paraffin embedded human hepatocarcinoma tissue using HP antibody (Center)
    • Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram) using HP antibody (Center)
    • Confocal immunofluoroscence analysis of MDA-MB435 cell using HP antibody (Center)
    • Western blot analysis of MDA-MB453, CEM, NCI-H460, HepG2, K562 cell line lysates (35ug/lane) using HP antibody (Center)
    Haptoglobin antibody [orb28600]

    ELISA, FACS, ICC, IF, IHC-P, WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    400 μl
  2. 4 images
    • Immunohistochemistry of paraffin-embedded human liver tissue using Haptoglobin antibody
    • Western blot aanalysis of Mouse stomach tissueusing Haptoglobin antibody
    • Immunohistochemistry of paraffin-embedded human small intestine tissue using Haptoglobin antibody
    • Immunohistochemistry of paraffin-embedded human ovarian cancer tissue using Haptoglobin antibody
    Haptoglobin antibody [orb242858]

    ELISA, WB, IHC

    Human, Mouse

    Rabbit

    Polyclonal

    100 μg, 50 μg
  3. 4 images
    • Immunohistochemical staining of paraffin embedded mouse hepatocarcinoma tissue using Haptoglobin antibody (primary antibody dilution at: 1:10-50)
    • Western blot analysis of 293 cell lysateusing Haptoglobin antibody (primary antibody dilution at: 1:1000)
    • Western blot analysis of human liver tissue lysateusing Haptoglobin antibody (antibody dilution at 1:1000)
    Haptoglobin antibody [orb39622]

    FC, IHC-P, WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    400 μl, 80 μl
  4. 3 images
    • Western Blot analysis of A:Hela whole cell lysate using Haptoglobin antibody
    • Immunohistochemical analysis of formalin-fixed and paraffin-embedded HSC3 xenograft using Haptoglobin antibody
    • Confocal immunofluorescence analysis of methanol-fixed HeLa using Haptoglobin antibody
    Haptoglobin antibody [orb74137] Custom conjugation service available

    IF, WB, IHC

    Human

    Rabbit

    Polyclonal

    100 μl
  5. 3 images
    • Western Blot analysis of A:Hela whole cell lysate using Haptoglobin antibody
    • Immunohistochemical analysis of formalin-fixed and paraffin-embedded Normal gastric using Haptoglobin antibody
    • Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa using Haptoglobin antibody
    Haptoglobin antibody [orb74138] Custom conjugation service available

    IF, WB, IHC

    Human

    Rabbit

    Polyclonal

    100 μl