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Catalog Number | orb1927722 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB24639 |
Tested applications | FC, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 40166 Da |
Target | This HAPLN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-38 amino acids from the N-terminal region of human HAPLN1. |
UniProt ID | P10915 |
NCBI | NP_001875.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Hyaluronan and proteoglycan link protein 1, Cartil Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
HAPLN1 Antibody (N-term) western blot analysis in MCF-7 (lane 1), Jurkat (lane 2), HepG2 (lane 3) cell line lysates (35 ug/lane).This demonstrates the HAPLN1 antibody detected the HAPLN1 protein (arrow).
HAPLN1 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
HAPLN1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HAPLN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.