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GSDMC Antibody

Catalog Number: orb1270600

DispatchUsually dispatched within 5-10 working days
$ 880.00
Catalog Numberorb1270600
CategoryAntibodies
DescriptionGSDMC Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IHC-P, WB
ReactivityHuman
IsotypeRabbit Ig
ImmunogenThis GSDMC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the Central region of human GSDMC.
Antibody TypePrimary Antibody
Concentrationbatch dependent
Form/AppearanceLiquid
ConjugationUnconjugated
MW58 kDa
TargetGSDMC
UniProt IDQ9BYG8
NCBIQ9BYG8
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied in PBS with 0.09% (W/V) sodium azide.
Alternative namesGasdermin-C, Melanoma-derived leucine zipper-conta
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NoteFor research use only
Application notesFor FACS starting dilution is: 1:25For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:50~100
Expiration Date12 months from date of receipt.
GSDMC Antibody

Overlay histogram showing U-2OS cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (OH191631) at 1/200 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG (1 ug/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.

GSDMC Antibody

Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: MKN45 whole cell lysate Lane 3: SK-MEL-2 whole cell lysate Lysates/proteins at 20 ug per lane.

GSDMC Antibody

GSDMC antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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