Cart summary

You have no items in your shopping cart.

GSDMC Antibody (Center)

Catalog Number: orb1938716

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1938716
CategoryAntibodies
DescriptionAffinity Purified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB27043
Tested applicationsFC, IHC-P, WB
ReactivityHuman
IsotypeRabbit IgG
Antibody TypePrimary Antibody
Dilution rangeWB: 1:2000, IHC-P: 1:50~100, FC: 1:25
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
ConjugationUnconjugated
MW57692 Da
TargetThis GSDMC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the Central region of human GSDMC.
UniProt IDQ9BYG8
NCBINP_113603.1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesGasdermin-C, Melanoma-derived leucine zipper-conta
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
GSDMC Antibody (Center)

All lanes: Anti-GSDMC Antibody (Center) at 1:2000 dilution. Lane 1: A375 whole cell lysate. Lane 2: MKN45 whole cell lysate. Lane 3: SK-MEL-2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

GSDMC Antibody (Center)

GSDMC antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSDMC antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

GSDMC Antibody (Center)

Overlay histogram showing U-2OS cells (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.