GRN Antibody

• Catalog Number: orb1248740
• Category: Antibodies
• Description: GRN Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: PQDTVCDLIQSK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 75kDa band observed in lysates of cell lines A431, A549 and HeLa (calculated MW of 63.5kDa according to NP_002078.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (He and Bateman, Cancer Res. 1999 Jul 1;59(13):3222-9. PMID: 10397269). Recommended concentration: 1-3ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 75 kDa
• Target: GRN
• UniProt ID: P28799
• NCBI: NP_002078.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GRN, granulin, GEP, GP88, PCDGF, PEPI, PGRN, PC ce
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 75kDa band observed in lysates of cell lines A431, A549 and HeLa (calculated MW of 63.5kDa according to NP_002078.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (He and Bateman, Cancer Res. 1999 Jul 1;59(13):3222-9. PMID: 10397269). Recommended concentration: 1-3ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248740 (1 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.