Granzyme B antibody (FITC)

• Catalog Number: orb699855
• Category: Antibodies
• Description: Mouse monoclonalantibody against Granzyme B conjugated to FITC
• Clonality: Monoclonal
• Clone Number: CLB-GB11
• Tested applications: FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG1
• Immunogen: Human NK cell line YT-INDY-derived granzyme B
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: Granzyme B
• Entrez: 3002
• UniProt ID: P10144
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-GZMB antibody, anti-HLP antibody, anti-CTLA1
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor intracellular staining of human lymphocytes stained using anti-human Granzyme B (CLB-GB11) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD3 negative Granzyme B positive lymphocytes (red-filled) from CD3 negative Granzyme B negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Granzyme B (CLB-GB11) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Granzyme B (CLB-GB11) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).