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GPR30 Rabbit Polyclonal Antibody

Catalog Number: orb499624

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb499624
CategoryAntibodies
DescriptionGPR30 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityMouse, Rat
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human GPR30 (21-120/375aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW42 kDa
TargetGPER1
UniProt IDQ99527
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesG Protein Coupled Receptor 30; G-protein coupled r
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NoteFor research use only
Expiration Date12 months from date of receipt.
GPR30 Rabbit Polyclonal Antibody

Blank control (blue line): A431 cells (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C). Primary Antibody (green line): Rabbit Anti-PR30 antibody (orb499624), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

GPR30 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GPR30) Polyclonal Antibody, Unconjugated (orb499624) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

GPR30 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GPR30) Polyclonal Antibody, Unconjugated (orb499624) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

GPR30 Rabbit Polyclonal Antibody

Sample: Lane 1: Placenta (Mouse) Lysate at 40 ug, Lane 2: Fetal brain (Mouse) Lysate at 40 ug, Lane 3: Large intestine (Mouse) Lysate at 40 ug, Lane 4: Uterus (Mouse) Lysate at 40 ug, Lane 5: Heart (Mouse) Lysate at 40 ug, Lane 6: Large intestine (Rat) Lysate at 40 ug, Lane 7: Uterus (Rat) Lysate at 40 ug, Lane 8: Heart (Rat) Lysate at 40 ug, Primary: Anti-GPR30 (orb499624) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 58 kD.

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