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Catalog Number | orb76741 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to gp91phox |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 54.1 |
Tested applications | ICC, IHC-P, WB |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | Partially Purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100) |
Concentration | 1mg/ml |
Dilution range | Optimal antibody concentration should be determined by titration, however as a guideline try;WB: Use at an assay dependent dilution. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).ICC/IF: Use a concentration of 1 µg/ml.IHC-P: Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol |
Conjugation | Unconjugated |
Target | NOX2/gp91phox |
Entrez | 1536 |
UniProt ID | P04839 |
Buffer/Preservatives | Purified antibody containing PBS 0.1% sodium azide |
Alternative names | CGD91-phox antibody;CYBB antibody;Cytochrome b(558 Read more... |
Note | For research use only |
Application notes | SP2/0 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of A431 cell line lysates using gp91phox antibody
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Bovine, Porcine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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