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Product Overview
Product Name GM-CSF antibody
Catalog Number orb303513
Species/HostRabbit
ReactivityHuman
ConjugationUnconjugated
Tested applicationsIHC-P, S-ELISA, WB
Immunogen Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGM-CSF
Target GM-CSF
Product Properties
Form/Appearance A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Concentration 0.1-1.0 mg/ml
Storage Store at 4°C for short term and at-20°C for long term storage.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity Purified by affinity chromatography
Uniprot ID P04141 (Human)
Product Description

Rabbit polyclonal antibody to GM-CSF

Application Notes
Dilution Range WB: 0.1-0.2 μg/ml, IHC-P: 0.25 μg/ml
Application Notes WB Notes: To detect hGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Notes: To detect hGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required.
IHC Notes: This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma.  The recommended concentration is 0.25 µg/mL with an overnight incubation at 4°C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.  Optimal concentrations and conditions may vary. 
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