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Catalog Number | orb1929732 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB21070 |
Tested applications | IHC-P, WB |
Predicted Reactivity | Porcine |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Dilution range | WB: 1:2000, WB: 1:1000, WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:1000, WB: 1:2000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 42064 Da |
Target | This GLUL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-96 amino acids from the N-terminal region of human GLUL. |
UniProt ID | P15104 |
NCBI | NP_001028216.1, NP_002056.2, NP_001028228.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Glutamine synthetase, GS, Glutamate decarboxylase, Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-GLUL Antibody (N-term) at 1:1000 dilution + Raji whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of lysates from Hela, Raji, Jurkat, HT-29 cell line (from left to right), using GLUL Antibody (N-term). Diluted at 1:2000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.
All lanes: Anti-GLUL Antibody (N-term) at 1:2000 dilution. Lane 1: mouse cerebellum lysates. Lane 2: Jurkat whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-GLUL Antibody (N-term) at 1:2000 dilution. Lane 1: Jurkat whole cell lysates. Lane 2: mouse cerebellum lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of lysates from Hela, HT-29, Jurkat cell line, mouse brain, mouse cerebellum, rat cerebellum tissue lysate (from left to right), using GLUL Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.
All lanes: Anti-GLUL Antibody (N-term) at 1:2000 dilution. Lane 1: mouse brain lysates. Lane 2: Hela whole cell lysates. Lane 3: Jurkat whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-GLUL Antibody (N-term) at 1:2000 dilution. Lane 1: HT-29 whole cell lysates. Lane 2: mouse cerebellum lysates. Lane 3: Raji whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.