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Glucoamylase antibody (Peroxidase)

Glucoamylase antibody (Peroxidase)

Catalog Number: orb344268

DispatchUsually dispatched within 5-10 working days
$ 292.00
Catalog Numberorb344268
CategoryAntibodies
DescriptionGlucoamylase antibody (Peroxidase)
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, WB
ReactivityOther
IsotypeIgG
ImmunogenGlucoamylase [Rhizopus]
Concentration1.0 mg/mL
Dilution rangeELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:2,500
Form/AppearanceLiquid (sterile filtered)
PurityGlucoamylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glucoamylase [Rhizopus]. Cross reactivity against Glucoamylase from other sources is unknown.
ConjugationHRP
UniProt IDP07683
StorageStore vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Alternative namesgoat anti-Glucoamylase Antibody HRP Conjugation, P
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NoteFor research use only
Application notesAnti-Glucoamylase peroxidase has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Glucoamylase [Rhizopus] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Expiration Date12 months from date of receipt.
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