GLO1 Antibody

• Catalog Number: orb1263741
• Category: Antibodies
• Description: GLO1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Monkey
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This GLO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-51 amino acids from the N-terminal region of human GLO1.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 21 kDa
• Target: GLO1
• UniProt ID: Q04760
• NCBI: Q04760
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Lactoylglutathione lyase, Aldoketomutase, Glyoxala
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of GLO1 antibody in HL60 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human brain tissue reacted with GLO1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of widr cells using GLO1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of GLO1 using rabbit polyclonal GLO1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GLO1 gene.

Confocal immunofluorescent analysis of GLO1 Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).