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Catalog Number | orb2292900 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant GCLC. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 3H1 |
Tested applications | ELISA, IF, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG1 Kappa |
Immunogen | GCLC (NP_001489, 528 a.a. ~ 637 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | EGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEMNYSLILKCNQIANELCECPELLGSAFRKVKYSGSKTDSSN |
NCBI | NP_001489 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Detection limit for recombinant GST tagged GCLC is approximately 0.03 ng/ml as a capture antibody.
GCLC monoclonal antibody (M01), clone 3H1 Western Blot analysis of GCLC expression in A-431.
GCLC monoclonal antibody (M01), clone 3H1. Western Blot analysis of GCLC expression in NIH/3T3.
GCLC monoclonal antibody (M01), clone 3H1. Western Blot analysis of GCLC expression in PC-12.
Immunofluorescence of monoclonal antibody to GCLC on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of GCLC expression in transfected 293T cell line by GCLC monoclonal antibody (M01), clone 3H1. Lane 1: GCLC transfected lysate (72.8 KDa). Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (37.84 KDa).