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Catalog Number | orb256552 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to GATA1 (phospho-S310) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Porcine, Primate, Rat, Sheep |
Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S310 of human GATA1 protein. The exact sequence is proprietary. |
Dilution range | WB: 1:500-1000, IF/ICC: 1:100-500 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | GATA1 |
Entrez | 100911167, 25172, 2623, 14460 |
UniProt ID | P17679, P43429, P15976 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | anti ERYF1 antibody, anti GF1 antibody, anti Eryth Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of GATA1 (Phospho-S310) expression in K562 (A) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 43 kD)
Immunofluorescent analysis of GATA1 (Phospho-S310) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ELISA, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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