GAPDH antibody

• Catalog Number: orb213967
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GAPDH
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Porcine, Primate, Rabbit, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GAPDH. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: GAPDH
• Entrez: 100042025, 2597, 14433, 24383
• UniProt ID: P16858, P04406, P04797
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti GAPD antibody, anti Glyceraldehyde-3-phosphat
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GAPDH expression in Hela (A), mouse lung (B), mouse kidney (C), rat kidney (D) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 36 kD)

Immunofluorescent analysis of GAPDH staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).