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Item 1 of 4
GALE Antibody (Center)
Catalog Number: orb1929726
| Catalog Number | orb1929726 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB21189 |
| Tested applications | FC, IF, IHC-P, WB |
| Predicted Reactivity | Mouse |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 38282 Da |
| Target | This GALE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142-171 amino acids from the Central region of human GALE. |
| UniProt ID | Q14376 |
| NCBI | NP_001121093.1, NP_000394.2, NP_001008217.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | UDP-glucose 4-epimerase, Galactowaldenase, UDP-N-a Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
GALE Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of GALE Antibody (Center) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Formalin-fixed and paraffin-embedded human skin tissue reacted with GALE Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of GALE Antibody (Center) in A375 cell line lysates (35 ug/lane). GALE (arrow) was detected using the purified Pab.
