GAD65 antibody

• Catalog Number: orb18998
• Category: Antibodies
• Description: Goat polyclonal antibody to GAD65
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:32000, WB: 0.5-1.5 μg/ml
• Conjugation: Unconjugated
• MW: 65.4; 65.4
• Target: GAD2 / GAD65
• Entrez: 2572
• Protein Sequence: TLEDNEERMSRLSK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti-65 kDa glutamic acid decarboxylase antibody,
• Note: For research use only
• Application notes: This antibody is expected to recognise both reported variants.ELISA: antibody detection limit dilution 1:16000.WB: Approx 65kDa band observed in Human Brain (Cerebral Cortex, Cerebellum and Hippocampus) lysates (calculated MW of 65.4kDa according to NP_000809.1). Recommended concentration: 0.3-1µg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human Brain (Hippocampus) lysate using GAD65 antibody

Immunohistochemical staining of Human colon using GAD65 antibody

Immunohistochemical staining of Human Cerebellum using GAD65 antibody