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Catalog Number | orb1954412 |
---|---|
Category | Proteins |
Description | FCGRT & B2M Heterodimer Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 45.3 kDa and the accession number is NP_004098.1&NP_004039.1. |
Tag | His, Avi |
Purity | 98.00% |
MW | 45.3 kDa (predicted) |
UniProt ID | NP_004098.1&NP_004039.1 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | FCGRT & B2M Heterodimer Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 45.3 kDa and the accession number is NP_004098.1&NP_004039.1. |
Expression Region | A DNA sequence encoding the extracellular domain (Met1-Ser297) of human FCGRT (NP_004098.1) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (NP_004039.1) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the human FCGRT/B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Ala 24 & Ile 21 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |