FANCC Antibody

• Catalog Number: orb1262178
• Category: Antibodies
• Description: FANCC Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This FANCC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 527-555 amino acids from the C-terminal region of human FANCC.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IF starting dilution is: 1:25For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 63 kDa
• Target: FANCC
• UniProt ID: Q00597
• NCBI: Q00597
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Fanconi anemia group C protein, Protein FACC, FANC
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IF starting dilution is: 1:25For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from HepG2, HL-60 cell line (from left to right), using FANCC Antibody.

Immunofluorescent analysis of U251 cells, using FANCC Antibody. Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

FANCC Antibody IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.