FABP2 Antibody

• Catalog Number: orb1247536
• Category: Antibodies
• Description: FABP2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-EGVEAKRIFKKD
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FABP2
• UniProt ID: P12104
• NCBI: NP_000125.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: FABP2, fatty acid binding protein 2, intestinal ,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:A very strong approx 16kDa band observed in Human Duodenum lysates (calculated MW of 15.2kDa according to NP_000125.2). Primary incubation was 1 hour. In transfected HEK293 transiently expressing FABP2 a band of approx 18kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.001ug/ml.Immunohistochemistry:In paraffin embedded Human Small Intestine shows strong staining of basement membrane and weaker staining of enterocytes. Recommended concentration: 2-3ug/ml.
• Expiration Date: 12 months from date of receipt.

HEK293 overexpressing FABP2 and probed with orb1247536 (mock transfection in first lane).

orb1247536 (2.5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247536 (0.001 ug/ml) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.