EREG Antibody (C-term)

• Catalog Number: orb1788028
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: 137-165 aa
• Dilution range: WB: 1:2000, WB: 1:1000, IHC-P: 1:25, FC: 1:25, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 19044 Da
• Target: This EREG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-165 amino acids from the C-terminal region of human EREG.
• UniProt ID: O14944
• NCBI: NP_001423.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Proepiregulin, Epiregulin, EPR, EREG
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-EREG Antibody (C-term) at 1:1000 dilution. Lane 1: A549 whole cell lysates. Lane 2: HepG2 whole cell lysates. Lane 3: U87-MG whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-EREG Antibody (C-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: A549 whole cell lysates. Lane 3: NCI-H1299 whole cell lysates. Lane 4: U-87 MG whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining EREG in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Overlay histogram showing HepG2 cells stained (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.

Overlay histogram showing Hela cells stained (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.