EpCAM antibody

• Catalog Number: orb214207
• Category: Antibodies
• Description: Rabbit Polyclonal antibody againts EpCAM
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD326. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IP: 1-10-1-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: EPCAM
• Entrez: 4072, 17075, 171577
• UniProt ID: Q99JW5, O55159, P16422
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Adenocarcinoma associated antigen antibody, a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD326 expression in HCT116 (A), CT26 (B), PC12 (C) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 40 kD)

Immunofluorescent analysis of CD326 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.