ENTPD2 Antibody

• Catalog Number: orb1262264
• Category: Antibodies
• Description: ENTPD2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This ENTPD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-113 amino acids from the N-terminal region of human ENTPD2.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54 kDa
• Target: ENTPD2
• UniProt ID: Q9Y5L3
• NCBI: Q9Y5L3
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Ectonucleoside triphosphate diphosphohydrolase 2,
• Note: For research use only
• Application notes: For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:2000 dilution Lane 1: H.heart lysate Lane 2: 293 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: H.brain whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:1000 dilution Lane 1: human heart lysate Lane 2: 293 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: human brain lysate Lane 5: human skeletal muscle lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of ENTPD2 Antibody in K562 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human brain tissue reacted with ENTPD2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.