ELP2 Antibody

• Catalog Number: orb1264532
• Category: Antibodies
• Description: ELP2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This ELP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-765 amino acids from the C-terminal region of human ELP2.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 93 kDa
• Target: ELP2
• UniProt ID: Q6IA86
• NCBI: Q6IA86
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Elongator complex protein 2, ELP2, SHINC-2, STAT3-
• Note: For research use only
• Application notes: For IF starting dilution is: 1:100For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Fluorescent confocal image of SY5Y cells stained with ELP2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ELP2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the ELP2 immunosignal mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000134759).

Western blot analysis of ELP2 antibody in NCI-H460 cell line lysates (35 ug/lane)

Flow cytometric analysis of NCI-H292 cells using ELP2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.