ELF3 Antibody

• Catalog Number: orb1260399
• Category: Antibodies
• Description: ELF3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ELF3 (NP_004424.3).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 45kDa
• Target: ELF3
• UniProt ID: P78545
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: ELF3, ERT, ESX, EPR-1, ESE-1
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ELF3 antibody (orb1260399) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse heart using ELF3 antibody (orb1260399) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF7 cells using ELF3 antibody (orb1260399). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 150 ug extracts of A549 cells using 3 ug ELF3 antibody (orb1260399). Western blot was performed from the immunoprecipitate using ELF3 antibody (orb1260399) at a dilution of 1:500. Detection: ECL Enhanced Kit. Exposure time: 30s.