EGFR-IN-48 is a potent, orally bioavailable EGFR inhibitor effective against resistant mutants including del19/T790M/C797S, with low nanomolar IC50 values. It demonstrates anti-proliferative activity in corresponding BaF3 and PC-9 cell lines. This compound is a valuable research tool for investigating mechanisms of EGFR inhibitor resistance and developing next-generation targeted therapies.