EGFR Antibody [Matuzumab], Rabbit IgG - Research Grade Biosimilar

• Catalog Number: orb1671957
• Category: Antibodies
• Description: EGFR Antibody [Matuzumab], Rabbit IgG - Research Grade Biosimilar
• Clonality: Recombinant
• Clone Number: Matuzumab
• Tested applications: Blocking, ELISA, FC, Functional Studies, IF, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: The parental mouse antibody was generated by immunizing BALB/c mice intraperitoneally with A431 cells in phosphate buffered saline (PBS). Later on the humanzied version of the antibody was created by grafting CDRs of the murine antibody onto human constant regions.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: C3
• UniProt ID: P00533
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: ErbB-1, ERBB1, HER1, EGF, Epidermal growth factor
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-EGFR antibody Matuzum34, HeLa (A) and A431 (B) cells were stained with unimmunized r antibody (black line) or the rmeric version of Matuzum6 - blue line at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed A431 cells with anti-EGFR antibody Matuzum34. Immunofluorescence analysis of unpermearaformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with the chimeric rsion of Matuzum6 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671957- DAPI- merged channels and an isotype control. The isotype control was stained with unimmunised r followed by Alexa Fluor® 488 secondary antibody.