EGFR Antibody [Matuzumab], Human IgG1 - Research Grade Biosimilar

• Catalog Number: orb1671958
• Category: Antibodies
• Description: EGFR Antibody [Matuzumab], Human IgG1 - Research Grade Biosimilar
• Target: C3
• Clonality: Recombinant
• Isotype: IgG1 kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: batch dependent
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Immunogen: The parental mouse antibody was generated by immunizing BALB/c mice intraperitoneally with A431 cells in phosphate buffered saline (PBS). Later on the humanzied version of the antibody was created by grafting CDRs of the murine antibody onto human constant regions.
• UniProt ID: P00533
• Tested applications: Blocking, ELISA, FC, Functional Studies, IF, WB
• Antibody Type: Primary Antibody
• Clone Number: Matuzumab
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ErbB-1, ERBB1, HER1, EGF, Epidermal growth factor
• Note: For research use only

Immunohistochemical staining of mouse skin tissue using anti-EGFR antibody. MatuzumAnti-EGFR staining of formaldehyde fixed paraffin embedded mouse skin tissue - at 40x magnification. The human IgG1-chimeric version of Matuzum5) was used to stain samples at a concentration of 5 ug/ml.

Immunohistochemical staining of rat stomach tissue using anti-EGFR antibody. MatuzumAnti-EGFR staining of formaldehyde fixed paraffin embedded rat stomach tissue - at 40x magnification. The human IgG1-chimeric version of Matuzum5 was used to stain samples at a concentration of 5 ug/ml.

Binding curve of anti-EGFR antibody Matuzum5 to EGFR-Fc fusion protein. ELISA Plate coated with human EGFR-Fc (Pr00117-10.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 100 to 0.1 ng/ml was performed using orb1671958. For detection- a 1:4000 dilution of HRP lnti-human kappa light chain antibody was used.