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Item 1 of 4
EEF1B2 Antibody (Center)
Catalog Number: orb1788440
| Catalog Number | orb1788440 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | FC, IF, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Immunogen | 54-86 aa |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:25, WB: 1:1000, IHC-P: 1:25, FC: 1:25 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 24764 Da |
| Target | This EEF1B2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 54-86 amino acids from the Central region of human EEF1B2. |
| UniProt ID | P24534 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Elongation factor 1-beta, EF-1-beta, EEF1B2, EEF1B Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of lysates from Hela, K562, MCF-7 cell line (from left to right), using EEF1B2 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H. brain section using EEF1B2 Antibody (Center). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Hela cells using EEF1B2 Antibody (Center) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Fluorescent image of Hela cells stained with EEF1B2 Antibody (Center). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
