EAAC1 Antibody: Biotin

• Catalog Number: orb149929
• Category: Antibodies
• Description: Mouse monoclonal antibody against SLC1A1 conjugated to Biotin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N180/41 (Formerly sold as S180-41)
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Rat
• Isotype: IgG1
• Immunogen: Fusion protein amino acids 1-524 (full length) of human EAAC1
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:100);optimal dilutions for assays should be determined by the user.
• Conjugation: Biotin
• MW: 60kDa
• Target: EAAT3
• Entrez: 6505
• UniProt ID: P43005
• NCBI: NP_004161.4
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: Excitatory amino-acid carrier 1 antibody, excitato
• Note: For research use only
• Application notes: 5 µg of SMC-406 was sufficient for immunoprecipitation of EAAC1 from 50 µg of rat brain lysate.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EAAC1 Monoclonal Antibody, Clone N180/41. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-EAAC1 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) EAAC1 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EAAC1 Monoclonal Antibody, Clone N180/41. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EAAC1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) EAAC1 Antibody. (D) Composite.

Western Blot analysis of Rat brain membrane lysate showing detection of EAAT3 protein using Mouse Anti-EAAT3 Monoclonal Antibody, Clone N180/41. Primary Antibody: Mouse Anti-EAAT3 Monoclonal Antibody at 1:1000.