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DYKDDDDK (FLAG) Positive Control Lysate

Catalog Number: orb348661

Product Overview
Product Name DYKDDDDK (FLAG) Positive Control Lysate
Catalog Number orb348661
Tested applicationsWB
Hazard InformationNon-Toxic
Target DYKDDDDK (FLAG) Positive Control Lysate
Product Properties
Form/Appearance Liquid: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
10% (v/v) Glycerol
Concentration 1.0 mg/ml
Storage Store vial at -70°C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.
Note For research use only.
Product Description

DYKDDDDK (FLAG) Positive Control Lysate

Application Notes
Application Notes ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). If dissociating conditions are desired, add reducing agent prior to heating. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 μl is recommended for SDS-PAGE. The product is supplied in 1X loading buffer and is ready-to-use.
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