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Item 1 of 4
DPT Antibody (Center)
Catalog Number: orb1929215
| Catalog Number | orb1929215 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB17950 |
| Tested applications | FC, IF, IHC-P, WB |
| Predicted Reactivity | Mouse, Porcine |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 24005 Da |
| Target | This DPT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-128 amino acids from the Central region of human DPT. |
| UniProt ID | Q07507 |
| NCBI | NP_001928.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Dermatopontin, Tyrosine-rich acidic matrix protein Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of DPT antibody (Center) in HL60 cell line lysates (35 ug/lane). DPT (arrow) was detected using the purified Pab.
Flow cytometric analysis of NCI-H292 cells using DPT Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescence analysis of DPT Antibody (Center) with paraffin-embedded human brain tissue.0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
Formalin-fixed and paraffin-embedded human brain with DPT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
