Dog IgG

• Catalog Number: orb2652712
• Category: Proteins
• Description: Dog IgG
• Tested applications: ELISA, IHC, SDS-PAGE, WB
• Concentration: 10.0 mg/mL
• Dilution range: ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
• Form/Appearance: Lyophilized
• Purity: Dog IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Dog IgG whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog IgG and anti-Dog Serum.
• Conjugation: Unconjugated
• Biological Origin: Dog
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dog IgG whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: Dog immunoglobulin G, Canine IgG
• Note: For research use only
• Application notes: Dog IgG whole molecule has been tested in SDS-Page can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 500 µL
• Expiration Date: 6 months from date of receipt.

Full-length coagulase protein binding to prothrombin, canine IgG, and complement C3. Data were obtained from three independent experiments. (A) ELISA results showing coagulase binding of canine IgG, Fc, and Fab. There was a significant interaction of treatment (IgG, Fc, and Fab) with the binding of coagulase protein (P = 0.010). All three treatments had significant differences between results at different concentrations (P < 0.001). For IgG concentrations of 10, 5, 2.5, and 1.25 µg/ml, binding was significantly different from that of the negative control (*, P < 0.001). For Fc and Fab concentrations of 5 and 10 µg/ml, binding was significantly different from that of the negative control (*, P < 0.001). The coagulase protein bound more IgG and Fc than Fab. At 5 and 10 µg/ml, IgG binding was significantly higher than that of Fab (P = 0.008), and binding of IgG and Fc was marginally different (P = 0.054).

SDS-PAGE of Dog IgG Whole Molecule. Lane 1: Dog IgG Whole Molecule Reduced (10 µg). Lane 2: Dog IgG Whole Molecule Reduced (5 µg). Lane 3: Dog IgG Whole Molecule Reduced (1.0 µg). Lane 4: Opal Prestained Molecular Weight Marker. Lane 5: Dog IgG Whole Molecule Non-Reduced (10 µg). Lane 6: Dog IgG Whole Molecule Non-Reduced (5 µg). Lane 7: Dog IgG Whole Molecule Non-Reduced (1.0 µg). 4-20% Gel, Coomassie Stained.