DNMT1 antibody

• Catalog Number: orb148092
• Category: Antibodies
• Description: Mouse monoclonal to DNMT1 (APC). Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6)..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4G11-C7
• Tested applications: ChIP, IHC, IP, WB
• Reactivity: Fish, Human, Mouse, Zebrafish
• Isotype: IgG1 Kappa
• Immunogen: Raised against a partial protein corresponding to amino acids 620-950 of human DNMT1
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), IHC (1:1000)
• Conjugation: APC
• MW: 180kDa
• Target: DNMT1
• Entrez: 1786
• UniProt ID: P26358
• NCBI: NP_001370
• Storage: Conjugated antibodies should be stored at 4°C
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Alternative names: DNA Mtase antibody, DNMT antibody, MCMT antibody,
• Note: For research use only
• Application notes: 2 µg/ml of SMC-201 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.